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rabbit anti human anti phosphorylated stathmin polyclonal antibody  (Bioss)


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    Structured Review

    Bioss rabbit anti human anti phosphorylated stathmin polyclonal antibody
    Sequences of real-time PCR primers.
    Rabbit Anti Human Anti Phosphorylated Stathmin Polyclonal Antibody, supplied by Bioss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+human+anti+phosphorylated+stathmin+polyclonal+antibody/Stathmin+(Ser16)+Polyclonal+Antibody/pmc05529897-51-124-132
    Average 90 stars, based on 1 article reviews
    rabbit anti human anti phosphorylated stathmin polyclonal antibody - by Bioz Stars, 2026-09
    90/100 stars

    Images

    1) Product Images from "Siva 1 inhibits proliferation, migration and invasion by phosphorylating Stathmin in ovarian cancer cells"

    Article Title: Siva 1 inhibits proliferation, migration and invasion by phosphorylating Stathmin in ovarian cancer cells

    Journal: Oncology Letters

    doi: 10.3892/ol.2017.6307

    Sequences of real-time PCR primers.
    Figure Legend Snippet: Sequences of real-time PCR primers.

    Techniques Used: Real-time Polymerase Chain Reaction, Sequencing

    Siva 1 enhances phosphorylation of Stathmin. (A) Siva 1 does not affect Stathmin mRNA level. (B and C) Overexpression of Siva 1 enhances phosphorylation of Stathmin and acetylation of α-tubulin, but did not markedly affect the expression level of Stathmin and α-tubulin detected by western blot. ***P<0.001 vs. pCMV group; ns, no significance.
    Figure Legend Snippet: Siva 1 enhances phosphorylation of Stathmin. (A) Siva 1 does not affect Stathmin mRNA level. (B and C) Overexpression of Siva 1 enhances phosphorylation of Stathmin and acetylation of α-tubulin, but did not markedly affect the expression level of Stathmin and α-tubulin detected by western blot. ***P<0.001 vs. pCMV group; ns, no significance.

    Techniques Used: Over Expression, Expressing, Western Blot

    Related Articles

    Blocking Assay:

    Article Title: Siva 1 inhibits proliferation, migration and invasion by phosphorylating Stathmin in ovarian cancer cells
    Article Snippet: Subsequent to quantification of the content using a bicinchoninic acid protein quantification kit (Wanleibio Co., Ltd.), the protein was isolated by SDS-PAGE using 40 μg protein in each lane and transferred to polyvinylidene fluoride (PVDF) membrane (EMD Millipore, Billerica, MA, USA). .. Subsequent to blocking with 5% skim milk (YILI, Hohhot, Inner Mongolia, China) at room temperature for 1 h, diluted by TBS Tween (TBST), the PVDF membrane was incubated with the following antibodies at 4°C overnight: rabbit anti-human anti-Siva 1 polyclonal antibody (dilution, 1:200; Santa Cruz Biotechnology, Inc., Dallas, TX, USA, cat. no., sc-48767), rabbit anti-human anti-cleaved caspase-3 polyclonal antibody (dilution, 1:1,000; Abcam, Cambridge, UK, cat. no., ab2302), goat anti-human anti-cleaved caspase-9 polyclonal antibody (dilution, 1:200; Santa Cruz Biotechnology, Inc., cat. no., sc-22182), rabbit anti-human anti-Bcl-2-like protein 4 (Bax) polyclonal antibody (dilution, 1:400; Boster, Hubei, Wuhan, China, cat. no., BA0315), rabbit anti-human anti-Bcl-2 polyclonal antibody (dilution, 1:400; Boster, cat. no., BA0412), rabbit anti-human anti-Stathmin polyclonal antibody (dilution, 1:500; Bioss Antibodies, Beijing, China, cat. no., bs-1902R), rabbit anti-human anti-phosphorylated Stathmin polyclonal antibody (dilution, 1:500; Bioss Antibodies, cat. no., bs-3431R), rabbit anti-human anti-α-tubulin polyclonal antibody (dilution, 1:1,000; Abcam, cat. no., ab178484) or mouse anti-human anti-acetyl-α-tubulin monoclonal antibody (dilution, 1:1,000; Abcam, cat. no., ab24610). .. Subsequent to washing with TBST 4 times for 5 min each, the PVDF membrane was incubated with goat anti-rabbit polyclonal IgG-horseradish peroxidase (HRP; dilution, 1:5,000; Wanleibio Co., Ltd., cat. no., WLA023), goat anti-mouse polyclonal IgG-HRP (dilution, 1:5,000; Wanleibio Co., Ltd., cat. no., WLA024) or donkey anti-goat polyclonal IgG-HRP (dilution, 1:5,000; Beyotime Institute of Biotechnology, Haimen, Jiangsu, China, cat. no., A0181) at 37°C for 45 min, followed treatment with a ECL reagent (Wanleibio Co., Ltd.).

    Membrane:

    Article Title: Siva 1 inhibits proliferation, migration and invasion by phosphorylating Stathmin in ovarian cancer cells
    Article Snippet: Subsequent to quantification of the content using a bicinchoninic acid protein quantification kit (Wanleibio Co., Ltd.), the protein was isolated by SDS-PAGE using 40 μg protein in each lane and transferred to polyvinylidene fluoride (PVDF) membrane (EMD Millipore, Billerica, MA, USA). .. Subsequent to blocking with 5% skim milk (YILI, Hohhot, Inner Mongolia, China) at room temperature for 1 h, diluted by TBS Tween (TBST), the PVDF membrane was incubated with the following antibodies at 4°C overnight: rabbit anti-human anti-Siva 1 polyclonal antibody (dilution, 1:200; Santa Cruz Biotechnology, Inc., Dallas, TX, USA, cat. no., sc-48767), rabbit anti-human anti-cleaved caspase-3 polyclonal antibody (dilution, 1:1,000; Abcam, Cambridge, UK, cat. no., ab2302), goat anti-human anti-cleaved caspase-9 polyclonal antibody (dilution, 1:200; Santa Cruz Biotechnology, Inc., cat. no., sc-22182), rabbit anti-human anti-Bcl-2-like protein 4 (Bax) polyclonal antibody (dilution, 1:400; Boster, Hubei, Wuhan, China, cat. no., BA0315), rabbit anti-human anti-Bcl-2 polyclonal antibody (dilution, 1:400; Boster, cat. no., BA0412), rabbit anti-human anti-Stathmin polyclonal antibody (dilution, 1:500; Bioss Antibodies, Beijing, China, cat. no., bs-1902R), rabbit anti-human anti-phosphorylated Stathmin polyclonal antibody (dilution, 1:500; Bioss Antibodies, cat. no., bs-3431R), rabbit anti-human anti-α-tubulin polyclonal antibody (dilution, 1:1,000; Abcam, cat. no., ab178484) or mouse anti-human anti-acetyl-α-tubulin monoclonal antibody (dilution, 1:1,000; Abcam, cat. no., ab24610). .. Subsequent to washing with TBST 4 times for 5 min each, the PVDF membrane was incubated with goat anti-rabbit polyclonal IgG-horseradish peroxidase (HRP; dilution, 1:5,000; Wanleibio Co., Ltd., cat. no., WLA023), goat anti-mouse polyclonal IgG-HRP (dilution, 1:5,000; Wanleibio Co., Ltd., cat. no., WLA024) or donkey anti-goat polyclonal IgG-HRP (dilution, 1:5,000; Beyotime Institute of Biotechnology, Haimen, Jiangsu, China, cat. no., A0181) at 37°C for 45 min, followed treatment with a ECL reagent (Wanleibio Co., Ltd.).

    Incubation:

    Article Title: Siva 1 inhibits proliferation, migration and invasion by phosphorylating Stathmin in ovarian cancer cells
    Article Snippet: Subsequent to quantification of the content using a bicinchoninic acid protein quantification kit (Wanleibio Co., Ltd.), the protein was isolated by SDS-PAGE using 40 μg protein in each lane and transferred to polyvinylidene fluoride (PVDF) membrane (EMD Millipore, Billerica, MA, USA). .. Subsequent to blocking with 5% skim milk (YILI, Hohhot, Inner Mongolia, China) at room temperature for 1 h, diluted by TBS Tween (TBST), the PVDF membrane was incubated with the following antibodies at 4°C overnight: rabbit anti-human anti-Siva 1 polyclonal antibody (dilution, 1:200; Santa Cruz Biotechnology, Inc., Dallas, TX, USA, cat. no., sc-48767), rabbit anti-human anti-cleaved caspase-3 polyclonal antibody (dilution, 1:1,000; Abcam, Cambridge, UK, cat. no., ab2302), goat anti-human anti-cleaved caspase-9 polyclonal antibody (dilution, 1:200; Santa Cruz Biotechnology, Inc., cat. no., sc-22182), rabbit anti-human anti-Bcl-2-like protein 4 (Bax) polyclonal antibody (dilution, 1:400; Boster, Hubei, Wuhan, China, cat. no., BA0315), rabbit anti-human anti-Bcl-2 polyclonal antibody (dilution, 1:400; Boster, cat. no., BA0412), rabbit anti-human anti-Stathmin polyclonal antibody (dilution, 1:500; Bioss Antibodies, Beijing, China, cat. no., bs-1902R), rabbit anti-human anti-phosphorylated Stathmin polyclonal antibody (dilution, 1:500; Bioss Antibodies, cat. no., bs-3431R), rabbit anti-human anti-α-tubulin polyclonal antibody (dilution, 1:1,000; Abcam, cat. no., ab178484) or mouse anti-human anti-acetyl-α-tubulin monoclonal antibody (dilution, 1:1,000; Abcam, cat. no., ab24610). .. Subsequent to washing with TBST 4 times for 5 min each, the PVDF membrane was incubated with goat anti-rabbit polyclonal IgG-horseradish peroxidase (HRP; dilution, 1:5,000; Wanleibio Co., Ltd., cat. no., WLA023), goat anti-mouse polyclonal IgG-HRP (dilution, 1:5,000; Wanleibio Co., Ltd., cat. no., WLA024) or donkey anti-goat polyclonal IgG-HRP (dilution, 1:5,000; Beyotime Institute of Biotechnology, Haimen, Jiangsu, China, cat. no., A0181) at 37°C for 45 min, followed treatment with a ECL reagent (Wanleibio Co., Ltd.).



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    rabbit anti human anti phosphorylated stathmin polyclonal antibody  (Bioss)
    90
    Bioss rabbit anti human anti phosphorylated stathmin polyclonal antibody
    Sequences of real-time PCR primers.
    Rabbit Anti Human Anti Phosphorylated Stathmin Polyclonal Antibody, supplied by Bioss, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+anti+human+anti+phosphorylated+stathmin+polyclonal+antibody/Stathmin+(Ser16)+Polyclonal+Antibody/pmc05529897-51-124-132
    Average 90 stars, based on 1 article reviews
    rabbit anti human anti phosphorylated stathmin polyclonal antibody - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Sequences of real-time PCR primers.

    Journal: Oncology Letters

    Article Title: Siva 1 inhibits proliferation, migration and invasion by phosphorylating Stathmin in ovarian cancer cells

    doi: 10.3892/ol.2017.6307

    Figure Lengend Snippet: Sequences of real-time PCR primers.

    Article Snippet: Subsequent to blocking with 5% skim milk (YILI, Hohhot, Inner Mongolia, China) at room temperature for 1 h, diluted by TBS Tween (TBST), the PVDF membrane was incubated with the following antibodies at 4°C overnight: rabbit anti-human anti-Siva 1 polyclonal antibody (dilution, 1:200; Santa Cruz Biotechnology, Inc., Dallas, TX, USA, cat. no., sc-48767), rabbit anti-human anti-cleaved caspase-3 polyclonal antibody (dilution, 1:1,000; Abcam, Cambridge, UK, cat. no., ab2302), goat anti-human anti-cleaved caspase-9 polyclonal antibody (dilution, 1:200; Santa Cruz Biotechnology, Inc., cat. no., sc-22182), rabbit anti-human anti-Bcl-2-like protein 4 (Bax) polyclonal antibody (dilution, 1:400; Boster, Hubei, Wuhan, China, cat. no., BA0315), rabbit anti-human anti-Bcl-2 polyclonal antibody (dilution, 1:400; Boster, cat. no., BA0412), rabbit anti-human anti-Stathmin polyclonal antibody (dilution, 1:500; Bioss Antibodies, Beijing, China, cat. no., bs-1902R), rabbit anti-human anti-phosphorylated Stathmin polyclonal antibody (dilution, 1:500; Bioss Antibodies, cat. no., bs-3431R), rabbit anti-human anti-α-tubulin polyclonal antibody (dilution, 1:1,000; Abcam, cat. no., ab178484) or mouse anti-human anti-acetyl-α-tubulin monoclonal antibody (dilution, 1:1,000; Abcam, cat. no., ab24610).

    Techniques: Real-time Polymerase Chain Reaction, Sequencing

    Siva 1 enhances phosphorylation of Stathmin. (A) Siva 1 does not affect Stathmin mRNA level. (B and C) Overexpression of Siva 1 enhances phosphorylation of Stathmin and acetylation of α-tubulin, but did not markedly affect the expression level of Stathmin and α-tubulin detected by western blot. ***P<0.001 vs. pCMV group; ns, no significance.

    Journal: Oncology Letters

    Article Title: Siva 1 inhibits proliferation, migration and invasion by phosphorylating Stathmin in ovarian cancer cells

    doi: 10.3892/ol.2017.6307

    Figure Lengend Snippet: Siva 1 enhances phosphorylation of Stathmin. (A) Siva 1 does not affect Stathmin mRNA level. (B and C) Overexpression of Siva 1 enhances phosphorylation of Stathmin and acetylation of α-tubulin, but did not markedly affect the expression level of Stathmin and α-tubulin detected by western blot. ***P<0.001 vs. pCMV group; ns, no significance.

    Article Snippet: Subsequent to blocking with 5% skim milk (YILI, Hohhot, Inner Mongolia, China) at room temperature for 1 h, diluted by TBS Tween (TBST), the PVDF membrane was incubated with the following antibodies at 4°C overnight: rabbit anti-human anti-Siva 1 polyclonal antibody (dilution, 1:200; Santa Cruz Biotechnology, Inc., Dallas, TX, USA, cat. no., sc-48767), rabbit anti-human anti-cleaved caspase-3 polyclonal antibody (dilution, 1:1,000; Abcam, Cambridge, UK, cat. no., ab2302), goat anti-human anti-cleaved caspase-9 polyclonal antibody (dilution, 1:200; Santa Cruz Biotechnology, Inc., cat. no., sc-22182), rabbit anti-human anti-Bcl-2-like protein 4 (Bax) polyclonal antibody (dilution, 1:400; Boster, Hubei, Wuhan, China, cat. no., BA0315), rabbit anti-human anti-Bcl-2 polyclonal antibody (dilution, 1:400; Boster, cat. no., BA0412), rabbit anti-human anti-Stathmin polyclonal antibody (dilution, 1:500; Bioss Antibodies, Beijing, China, cat. no., bs-1902R), rabbit anti-human anti-phosphorylated Stathmin polyclonal antibody (dilution, 1:500; Bioss Antibodies, cat. no., bs-3431R), rabbit anti-human anti-α-tubulin polyclonal antibody (dilution, 1:1,000; Abcam, cat. no., ab178484) or mouse anti-human anti-acetyl-α-tubulin monoclonal antibody (dilution, 1:1,000; Abcam, cat. no., ab24610).

    Techniques: Over Expression, Expressing, Western Blot